Platinum complexes inhibit ATP-driven basic dye uptake in lysosomes from rat liver
نویسندگان
چکیده
منابع مشابه
Uptake of imipramine in rat liver lysosomes in vitro and its inhibition by basic drugs.
We investigated the uptake of imipramine (IMP) in highly purified lysosomes from rat liver and its inhibition by a variety of basic drugs in vitro. The uptake of [(3)H]IMP into lysosomes peaked in less than 20 s, showing little temperature dependency or countertransport phenomena. It was accelerated by increase of extralysosomal pH, stimulated by Mg(2+)-ATP in KCl buffer, and suppressed by acid...
متن کاملIsolating Lysosomes from Rat Liver.
This protocol describes the generation of a fraction enriched in lysosomes from rat liver. The lysosomes are rapidly isolated using density-gradient centrifugation with gradient media that retain the osmolarity of the lysosomes such that they are functional and can be used in in vitro assays.
متن کاملEnrichment of bis-(monoacylglyceryl) phosphate in lysosomes from rat liver.
The subcellular distribution in rat liver of a phospholipid identified as bis-(monoacylglyceryl) phosphate (bis(MAG)P) has been investigated. In liver from untreated animals, 48 % of bis(MAG)P was recovered in a crude mitochondrial fraction where it was enriched 2.8-fold over the homogenate in terms of protein. When liver from animals who had received Triton WR-1339 by injection 3 days earlier ...
متن کاملCystine transport in purified rat liver lysosomes.
Amino acid efflux from highly purified rat liver lysosomes exposed to the methyl ester derivatives of leucine, methionine, tyrosine and cystine was examined. The lysosomal efflux of leucine, methionine and tyrosine was unaffected by the presence of MgATP, whereas cystine efflux was stimulated by MgATP. Exposure of lysosomes to 2 mM-MgATP resulted in lysosomal acidification and a 0.5 pH unit inc...
متن کاملThe interaction of tributyllead with lysosomes from rat liver.
The interactions of tributyllead with lysosomes from rat liver have been studied. It results that the organometal compound induces a fast alkalinization in energized lysosomes. The interpretation is that the compound is a potent proton carrier. This function could explain the toxicity, in particular at neurological level of the compound.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: FEBS Letters
سال: 1982
ISSN: 0014-5793
DOI: 10.1016/0014-5793(82)80731-x